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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis <t>of</t> <t>p-IRF3</t> expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.
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( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Journal: International Journal of Nanomedicine

Article Title: A Multifunctional Metal-Based Nanomedicine for Copper Sequestration, in situ Toxification, and Immune Activation Against Metastatic Cancer

doi: 10.2147/IJN.S580749

Figure Lengend Snippet: ( A ) The intracellular HMGB1 after different treatments toward 4T1 cells. ( B ) Immunofluorescence images showing CRT expression on the cell membrane of 4T1 cells, and ( C ) corresponding fluorescence quantification. Scale bar = 50 μm. ( D ) Immunofluorescence images of CD206 expression in RAW264.7 macrophages, and ( E ) quantitative analysis. Scale bar = 50 μm. ( F ) Immunofluorescence images of CD86 expression in RAW264.7 macrophages, and ( G ) quantitative analysis. Scale bar = 50 μm. ( H ) Secretion levels of anti-inflammatory cytokine IL-10 and ( I ) pro-inflammatory cytokine TNF-α from RAW264.7 cells after various treatments. ( J ) Western blot analysis of p-IRF3 expression in RAW264.7 cells, and ( K ) quantification of p-IRF3 band intensity. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: The calreticulin (CRT), CD206, CD86, and phospho-interferon regulatory factor 3 (p-IRF3) antibody were purchased from Proteintech Group, Inc. (Wuhan, China).

Techniques: Immunofluorescence, Expressing, Membrane, Fluorescence, Analysis, Western Blot